Sweetpotato is an important food and cash crop in sub-Saharan Africa (SSA), because it is tolerant to adverse growing conditions and has a short growing period. However, sweetpotato farmers in Africa have problems accessing healthy planting material. Often, they rely on sprouts from un-harvested roots left in the ground from the previous season. Such planting material may have high levels of sweetpotato virus infection, hence leading to considerable reduction in yields. Providing adequate supplies of improved quality of planting material at the right time is vital for the increased production and utilization of sweetpotato. Notably, in the Lake Zone of Tanzania, sweetpotato virus diseases exert enormous pressure on the growing crop. The use of pathogen tested tissue culture plantlets as source material is one approach to improving the quality of planting material available to farmers. Tissue culture is a promising technology, however it is costly and some critical steps are required for the successful transfer of in vitro plantlets to field conditions for further multiplication. There had been limited efforts to test the use of this method at large scale as part of a sweetpotato seed system intervention. Between 2010 and 2012 the International Potato Center (CIP) through the Sweetpotato Action for Security and Health in Africa (SASHA) project, partnered with the Lake Zone Agricultural Research and Development Institute (LZARDI), the Kenya Plant Health and Inspectorate Service (KEPHIS), Catholic Relief Services (CRS) and CRS-affiliated community development organizations to initiate an intervention for the promotion of timely dissemination of quality planting material. The intervention, popularly referred to as ‘Marando Bora’ involved the successful cross border transfer of 35,000 sweetpotato tissue culture plantlets by road from Nairobi (Kenya) to Bukoba (Tanzania), a distance of about 1,500km, and the subsequent acclimatization (or hardening)process. This manual is based on the experiences and lessons from the implementation of the SASHA Marando Bora project in Tanzania. It seeks not only to bridge the gap in information and capacity, but also to generate interest and guide similar efforts elsewhere in SSA. It shares the hands-on practices for: the initiation and mass propagation of tissue culture plantlets, transportation, hardening and field establishment. Factors that increase the survival and growth of plantlets into field plants are highlighted. This manual is intended for scientists and technicians who are responsible for the successful transfer of sweetpotato tissue culture plantlets for field multiplication and who are working in limited resource settings in SSA. The manual will also provide seed system practitioners in general, an overview of the tissue culture based procedures required for germplasm management and exchange so as to ensure that the quality of seed available to farmers is improved.